Jacobs began his musical career as a boy chorister at the Cathedral of Ghent. Later he studied classical philology at the University of Ghent while continuing to sing in Brussels and in The Hague.
The Kuijken brothers, Gustav Leonhardt and Alfred Deller all encouraged him to pursue a career as a countertenor, and he quickly became known as one of the best of his time. He recorded a large amount of less-known Baroque music by such composers as Antonio Cesti, d'India, Ferrari, Marenzio, Lambert, Guédron, William Lawes and others. He also sang in much-acclaimed recordings of the major works of Bach (such as the St Matthew Passion led by Gustav Leonhardt and Philippe Herreweghe).

Jacobs regularly conducts such orchestras and ensembles as the Concerto Köln, the Orchestra of the Age of Enlightenment, Akademie für Alte Musik Berlin, Freiburger Barockorchester, Nederlands Kamerkoor and RIAS Kammerchor for recordings and concert tours. Read more on Last.fm. User-contributed text is available under the Creative Commons By-SA License; additional terms may apply.